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database management
 

Methods

 

 

 

Tissue Processing

Generation of Template DNA
In Situ Hybridization
Imaging
Database management
BGEM Website Contruction
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Database management
 
To maintain the database with accurate bioinformatics, we employ an automatic search script written by Perdeep Mehta, Ph. D. at the Hartwell Center for Bioinformatics and Biotechnology. This script compares all gene entries in the BGEM database against the latest download of gene information from NCBI ( National Center for Biotechnology Information). Each week BLAST searches are performed using the nucleotide sequence of the DNA templates used for generating riboprobes for hybridization analysis. The BLAST retrieves the corresponding Genbank entry, GeneID, Unigene #, alternate gene symbols, Chromosome and cytogenetic information and gene ontology information. Any change detected between our database and the NCBI is documented and the BGEM database is altered to reflect the latest bioinformatics available at NCBI.




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