| 1. |
Equipment: |
| |
|
a. |
Images are captured using a Zeiss Stemi 11 Stereomicroscope
set at low magnification sufficient to capture the entire section
of coronal and saggital tissues. |
| |
|
b. |
Darkfield images are illuminated using a 3” MVI edge illuminator
connected to a 15 W halogen light power source set on maximum
for all photography. Reference cresyl violet images are captured
using the brightfield settings on the microscope. |
| |
c. |
Images are captured
using a Photometrics Coolsnap es Monochrome ADC 20 Mhz Camera
made by Sony with a 1300 x 1040 High resolution Interline
chip directly mounted to the Zeiss Stemi 11 Stereomicroscope. |
| |
d. |
MetaView (Version 6.4a) software is used to control the camera
and to properly orient each image for display on the website |
| |
e. |
Darkfield images are captured and the exposure time of capture
using the Metaview software is logged into our project management
database. Exposure time varies for each microscope slide depending
on the signal strength. We attempt to capture a field identical
to what is observed on the slide through the eyepieces of the
microscope. Image capture time ranges from <30 ms to >130
ms. |
| |
f. |
Images are captured as grayscale .TIFF files and converted
to .JPEG files for permanent storage. |
| 2. |
Image processing: Images are imported into Adobe
Photoshop 7.0 to optimize brightness, contrast and gamma levels
and to remove debris from the background using the healing brush
tool. |
| 3. |
Images are then converted to intermediate and
thumbnail sizes using Adobe Photoshop 7.0. for display on the
website: |
| |
|
a.
b.
c. |
Full size: 1392
x 1040 pixels 2
Intermediate size:
500 x 328 pixels 2
Thumbnail size:
200 x 130 pixels 2 |
| 4. |
Image upload: All images are uploaded to a holding
folder on the server for display on the website. Images are
uploaded to the website server using FTP Voyager software version
10.0 by RhinoSoft.com ( Helenville , WI , 53137 ). |
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